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talon resin  (TaKaRa)


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    Structured Review

    TaKaRa talon resin
    Talon Resin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1957 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/talon+resins/TALON+Metal+Affinity+Resin/pmc13089183-296-6-8
    Average 99 stars, based on 1957 article reviews
    talon resin - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Mapping the conformational landscape of the stimulatory heterotrimeric G protein
    Article Snippet: .. The lysate was centrifuged at 20,000 g for 30 minutes, and the resulting supernatant was incubated with TALON resins (Takara Bio) for 3 h at 4 °C with gentle mixing. ..

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml −1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co 2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications.
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml−1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Article Title: GluDs are ionotropic dopamine receptors tuned by G-proteins
    Article Snippet: .. The supernatant was collected by centrifugation (2,500 xg for 20 mins at 4 °C) and incubated with 5 mL of Talon resins (TaKaRa, 635503) for an hour. ..

    Gentle:

    Article Title: Mapping the conformational landscape of the stimulatory heterotrimeric G protein
    Article Snippet: .. The lysate was centrifuged at 20,000 g for 30 minutes, and the resulting supernatant was incubated with TALON resins (Takara Bio) for 3 h at 4 °C with gentle mixing. ..

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml −1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co 2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications.
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml−1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Transformation Assay:

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml −1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co 2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications.
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml−1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Protein Purification:

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml −1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co 2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications.
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml−1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Affinity Chromatography:

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml −1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co 2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Article Title: StayGold variants for molecular fusion and membrane-targeting applications.
    Article Snippet: StayGold, in a pET-47b(+) vector (Novagen) carrying ampicillin resistance and an HRV 3C-cleavable N-terminal polyhistidine tag, was expressed in E. coli (BL21(DE3)). .. Transformed E. coli was incubated at 25 °C in an LB medium containing 20 μg ml−1 kanamycin with gentle shaking (63 r.p.m.) for 5 d. Protein purification by Co2+ affinity chromatography was performed using TALON resins (Clontech). ..

    Binding Assay:

    Article Title: Computational scoring and experimental evaluation of enzymes generated by neural networks
    Article Snippet: .. Talon resins (cat. no. 635653, Takara Bio) were washed twice with a binding buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 10 mM imidazole, pH 7.4) and then suspended in the same volume of binding buffer as the resin bed amount. .. Talon resin (50 μl) was loaded into Pierce microspin columns (cat. no. 89879, ThermoFisher).

    other:

    Article Title: Combined preparations of PKM2 modulators and HMGB1
    Article Snippet: The cell pellet was homogenized and purified via IMAC and TALON (Clontech) Resins and eluted using imidazole.

    Article Title: Two molecule force spectroscopy on ligand-receptor interactions.
    Article Snippet: Nanoscale rsc.li/nanoscale ISSN 2040-3372 PAPER Hongbin Li et al. Two molecule force spectroscopy on ligand–receptor interactions Volume 15 Number 41 7 November 2023 Pages 16503-16804

    Article Title: Computational scoring and experimental evaluation of enzymes generated by neural networks.
    Article Snippet: All cells were cultivated in 24-well plates in an Eppendorf ThermoMixer C. For MDH expression, cells were grown for 4 h at 37 °C, followed by overnight growth at 16 °C while shaking at 200 rpm.

    Centrifugation:

    Article Title: GluDs are ionotropic dopamine receptors tuned by G-proteins
    Article Snippet: .. The supernatant was collected by centrifugation (2,500 xg for 20 mins at 4 °C) and incubated with 5 mL of Talon resins (TaKaRa, 635503) for an hour. ..



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